Dorothy Stafford, Lady Stafford (1 October 1526 – 22 September 1604) was an English noblewoman, and an influential person at the court of Queen Elizabeth I of England, to whom Dorothy served as Mistress of the Robes.
Dorothy was the second wife of Sir William Stafford, widower of Mary Boleyn.
Dorothy and her family were forced to seek exile in Geneva during the reign of Mary I due to their Protestant religion.
The Protestant reformer John Calvin stood as godfather to her youngest son.
Through her maternal grandmother, Margaret Pole, 8th Countess of Salisbury, Dorothy had a claim to the English throne.
Her paternal grandfather's lineage could be traced back to Anne of Gloucester, this collective ancestry making Dorothy the first noble to descend from all of the younger surviving sons of Edward III.