John Guthrie (born 10 September 1795 in Newark, Nottinghamshire; died 6 July 1865 in Clifton, Bristol) was an English first-class cricketer associated with Cambridge University.
He is recorded as a batsman in two matches in 1819, totalling 32 runs with a highest score of 22.Guthrie was educated at Westminster School and Trinity College, Cambridge.
After graduating from Cambridge he became a Church of England priest and after other posts he was vicar of Calne, Wiltshire, from 1835 until his death.
In Calne he and his wife founded a local school, the Guthrie School.
He was appointed a canon of Bristol Cathedral in 1858 and was the first chairman of council of Clifton College, founded in 1862.