Afterwards he studied bacteriology with Pierre Paul Émile Roux at the Pasteur Institute.
In 1894 he received his doctorate, and he later served as chief of Jean Alfred Fournier's laboratory at the HĂ´pital Saint-Louis.
In 1904, Sabouraud introduced radiological treatment against ringworm of the scalp.
He was well known for his knowledge of scalp diseases, and had a clinic which attracted patients from all over the world.He invented a method to select fungi with a medium of low pH and a rather high concentration of sugar.
This medium, called Sabouraud agar is named after him.With Ferdinand-Jean Darier (1856–1938) and Henri Gougerot (1881–1955), he was editor of an eight-volume encyclopedia of dermatology titled "Nouvelle Pratique Dermatologique".