Pavel Tlustoš (born 27 February 1955 in Nové Mesto na Morave, Czechoslovakia) is a Czech agricultural chemist.
From 2000-10 he was Vice Dean of the Faculty of Agricultural Biology, Food and Natural Resources of the Czech University of Life Sciences Prague (CULS), and 2010-February 2018 he was the Dean of the faculty.
Since March 2018 he is again Vice Dean and responsible for international relations the faculty.