In 2009, she was appointed as the Shadow Minister for Innovation, Industry, Science and Research.
Mirabella narrowly lost her seat of Indi at the 2013 federal election to independent candidate Cathy McGowan.
She also resigned from the Coalition frontbench shortly prior to conceding defeat.
She subsequently attempted to regain the seat, standing again for the Liberal Party at the 2016 election, but was defeated by McGowan again on an increased margin.